Insertion junction: LMJ.RY0402.236856_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.236856
Chromosome:chromosome 4
Location:4022612
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre04.g232402 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCAGTGGGTCGTAGTCGGGTGGTGATTTG
Internal bar code:CGCGTGTGGGGGTGTACATGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:556
LEAP-Seq percent confirming:98.2955
LEAP-Seq n confirming:1038
LEAP-Seq n nonconfirming:18
LEAP-Seq n unique pos:25

Suggested primers for confirmation by PCR

Suggested primer 1:CCTTGCTCAACAGGAATGGT
Suggested primer 2:CTGTCCATCTATCCGCCAGT