| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.236858 |
| Chromosome: | chromosome 3 |
| Location: | 4375901 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g175351 | LMR2 | (1 of 14) PF01476 - LysM domain (LysM); Predicted protein with LysM domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGCTTCGGGTTCGTGCACCAAGGGCTGCA |
| Internal bar code: | TCAGTTGCAGCCCAGCGAGCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 647 |
| LEAP-Seq percent confirming: | 85.3686 |
| LEAP-Seq n confirming: | 9242 |
| LEAP-Seq n nonconfirming: | 1584 |
| LEAP-Seq n unique pos: | 51 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATAAGGTCTTGCCCGTGTG |
| Suggested primer 2: | TCCCGACATTCCTTAACTGC |