Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.236879 |
Chromosome: | chromosome_1 |
Location: | 4209690 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre01.g028000 | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | AACCCAAAGTGGTTGTGTCAGTGGTTGCAA |
Internal bar code: | TGCCGGAGTCATGGCATAGTGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 444 |
LEAP-Seq percent confirming: | 99.0521 |
LEAP-Seq n confirming: | 836 |
LEAP-Seq n nonconfirming: | 8 |
LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CCCAAACTTCTCTGCCCATA |
Suggested primer 2: | GCAGTCTTCTCCCACTTTGC |