| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.236879 |
| Chromosome: | chromosome_11 |
| Location: | 2832150 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre11.g477550 | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TCCTCGGAGCCAGGCGCCGCGCCAGCAGTC |
| Internal bar code: | ACCGCGTCCCCGCTCCGAGGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 652 |
| LEAP-Seq percent confirming: | 99.0458 |
| LEAP-Seq n confirming: | 5086 |
| LEAP-Seq n nonconfirming: | 49 |
| LEAP-Seq n unique pos: | 41 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGCTTAACTCACGGGGACA |
| Suggested primer 2: | TTCTGATGGTCGATTCTCCC |