| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.236887 |
| Chromosome: | chromosome_10 |
| Location: | 4250879 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre10.g450350 | IFT25,FAP232 | Intraflagellar transport protein 25 | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GTCGGTGAGTGCGCGTGTGCTTCCCCTCCT |
| Internal bar code: | TATTCGTTTCCTCGAAGGTTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 652 |
| LEAP-Seq percent confirming: | 99.5686 |
| LEAP-Seq n confirming: | 13156 |
| LEAP-Seq n nonconfirming: | 57 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTAAGGCATTGCTGGGTAGC |
| Suggested primer 2: | TTCCTTTCCGCTCTGGTCTA |