| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.236895 |
| Chromosome: | chromosome 3 |
| Location: | 9093751 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g211073 | (1 of 2) K14497 - protein phosphatase 2C (PP2C) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAACAGCGAAAACGAAGCGCGGGGGGGAG |
| Internal bar code: | AATGTTCTAAGTATTTAACTGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 462 |
| LEAP-Seq percent confirming: | 98.2655 |
| LEAP-Seq n confirming: | 1303 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 44 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTGTCGACTGCTTGTCACC |
| Suggested primer 2: | CCCTGCGGTATACACGTTCT |