| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.236903 |
| Chromosome: | chromosome_10 |
| Location: | 4153199 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre10.g449750 | PKS1 | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CCACAAGAGGCTGGCGGCAGCAGCGCGGGT |
| Internal bar code: | TTTACTCCACAGCGTCATGACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 538 |
| LEAP-Seq percent confirming: | 99.7894 |
| LEAP-Seq n confirming: | 4265 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTTGCTGCTGTTGCTGTTG |
| Suggested primer 2: | TATGCGCTTCTGCCATACAG |