Insertion junction: LMJ.RY0402.236918_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.236918
Chromosome:chromosome 7
Location:1035387
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g319550 (1 of 1) PF10442 - FIST C domain (FIST_C) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGGCTCAACAGGGCTTCGGCATTATCGGT
Internal bar code:GAGATCGGTTTGCCGCGCAATT

Confirmation - LEAP-Seq

LEAP-Seq distance:545
LEAP-Seq percent confirming:36.3636
LEAP-Seq n confirming:160
LEAP-Seq n nonconfirming:280
LEAP-Seq n unique pos:27

Suggested primers for confirmation by PCR

Suggested primer 1:TGTTTGGTCCTCCTAATGCC
Suggested primer 2:ACTGAACTTGTGGGACGGAC