| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.236929 |
| Chromosome: | chromosome 16 |
| Location: | 4900846 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g685750 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCATAGGGTGTGCTACCGGTGTTACTTTT |
| Internal bar code: | TGGCCTTGCCCGGTGTCAAAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 387 |
| LEAP-Seq percent confirming: | 94.2448 |
| LEAP-Seq n confirming: | 3881 |
| LEAP-Seq n nonconfirming: | 237 |
| LEAP-Seq n unique pos: | 48 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAACAAAGTGGAGAGGAGCG |
| Suggested primer 2: | ACATCTGCAATATCCCGCTC |