Insertion junction: LMJ.RY0402.236929_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.236929
Chromosome:chromosome_16
Location:4900846
Confidence (%):95
Locus systematic id Locus common name Defline Orientation Feature
Cre16.g685750 antisense 3'UTR

Insertion site details

Flanking sequence (orientation from cassette outwards):CCTACTGCAGCACAGCACAGCACTCAAGCA
Internal bar code:CTCGGTGTGAGGTTAGCGGTTT

Confirmation - LEAP-Seq

LEAP-Seq distance:589
LEAP-Seq percent confirming:99.3451
LEAP-Seq n confirming:6826
LEAP-Seq n nonconfirming:45
LEAP-Seq n unique pos:71

Suggested primers for confirmation by PCR

Suggested primer 1:ACATCTGCAATATCCCGCTC
Suggested primer 2:GAACAAAGTGGAGAGGAGCG