| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.236942 |
| Chromosome: | chromosome_1 |
| Location: | 1777083 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g009550 | NAT6 | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TACGATCTAGCGCTCCGTAGATATGGTGGT |
| Internal bar code: | CAGAGGCCCGCAAATCGGCAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 545 |
| LEAP-Seq percent confirming: | 90.9091 |
| LEAP-Seq n confirming: | 20 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAAGAGCTCTGAGCCAGGT |
| Suggested primer 2: | TCCGCTGTAACATTATGGCA |