Insertion junction: LMJ.RY0402.236949_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.236949
Chromosome:chromosome 17
Location:5743688
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre17.g739150 CTPA5,TSP5 (1 of 8) 3.4.21.102 - C-terminal processing peptidase / Tsp protease; putative C-terminal peptidase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATGAATGTAACCCCCCCAGTCGCCAGCCGA
Internal bar code:CCGTTCGACCATGTGCTTCGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:587
LEAP-Seq percent confirming:99.0888
LEAP-Seq n confirming:3045
LEAP-Seq n nonconfirming:28
LEAP-Seq n unique pos:45

Suggested primers for confirmation by PCR

Suggested primer 1:AGCGTGTGGACACTAATCCC
Suggested primer 2:AACTCAAACCCACAAGTGCC