| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.236954 |
| Chromosome: | chromosome 3 |
| Location: | 9087315 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g210961 | (1 of 1) PTHR10174:SF127 - PHOSPHATIDYLINOSITOL TRANSFER PROTEIN PDR16-RELATED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGTGGGTGGGAGCTGGCGCGAGTCCCGA |
| Internal bar code: | TACGGTCTTGGACGACTTTGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 782 |
| LEAP-Seq percent confirming: | 2.32943 |
| LEAP-Seq n confirming: | 1519 |
| LEAP-Seq n nonconfirming: | 63690 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTGGTGTTTTAGCAGGCAG |
| Suggested primer 2: | CCTGACGAGACAGCAAATCA |