| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.236968 |
| Chromosome: | chromosome 1 |
| Location: | 5799327 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g041426 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GACATCCCCACACACCCTGTTCATAAGGAC |
| Internal bar code: | GCTGTGAGCGCTACCGTTAAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 629 |
| LEAP-Seq percent confirming: | 69.9566 |
| LEAP-Seq n confirming: | 1772 |
| LEAP-Seq n nonconfirming: | 761 |
| LEAP-Seq n unique pos: | 43 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATCAAGGTGCTGCGAGAGT |
| Suggested primer 2: | GCGCTTGACGTGCAGTATTA |