Insertion junction: LMJ.RY0402.236974_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.236974
Chromosome:chromosome 6
Location:6457936
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g292750 (1 of 2) PF05694 - 56kDa selenium binding protein (SBP56) (SBP56) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCCTGTTCTTCGTGGTTGTACGGCGCGGT
Internal bar code:CCGTCTGCCGGGACCTGGAGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:651
LEAP-Seq percent confirming:96.1397
LEAP-Seq n confirming:523
LEAP-Seq n nonconfirming:21
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:CAGGGTAAGGCACCAGTGAT
Suggested primer 2:GGATAGTTCGCAATGGGAGA