| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.236981 |
| Chromosome: | chromosome 1 |
| Location: | 2670593 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g015600 | (1 of 1) PTHR23346//PTHR23346:SF7 - TRANSLATIONAL ACTIVATOR GCN1-RELATED // TRANSLATIONAL ACTIVATOR GCN1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGAGAGCACGCGCGCGTGTGACCACTTTT |
| Internal bar code: | GTACCACGTACTGGTTTAAACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 675 |
| LEAP-Seq percent confirming: | 99.4067 |
| LEAP-Seq n confirming: | 9382 |
| LEAP-Seq n nonconfirming: | 56 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGCTCTGCATTGTTGTCTC |
| Suggested primer 2: | GCTGCTCAAGCCCTTCAC |