| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.237029 |
| Chromosome: | chromosome 16 |
| Location: | 1336474 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g651801 | (1 of 7) IPR000104//IPR020683 - Antifreeze protein, type I // Ankyrin repeat-containing domain | 5'UTR/CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCGACCACCAACGGCTAGGCCTCCGGACG |
| Internal bar code: | TTGGACCGAGCCTTACCAAGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 544 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 198 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCACAGCCAGGAGGTAGT |
| Suggested primer 2: | GGGAAGATCGAGGAATGACA |