Insertion junction: LMJ.RY0402.237051_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.237051
Chromosome:chromosome 2
Location:400660
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g076000 LAT3,STK1,STPK1,SNRK2K (1 of 587) 2.7.11.1 - Non-specific serine/threonine protein kinase / Threonine-specific protein kinase; snRK1 family in Chlamydomonas, subgroup 2; Latrunculin-B sensitive 3 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCACCCCCCAGCTGCGGTGTGGTGCTGTA
Internal bar code:TTAGAAGTCGACGAATAGGGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:431
LEAP-Seq percent confirming:98.75
LEAP-Seq n confirming:2212
LEAP-Seq n nonconfirming:28
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:GCTTCACCTCGTACAGGCTC
Suggested primer 2:GCCAAACTTCATGGGTGAGT