| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.237053 |
| Chromosome: | chromosome 3 |
| Location: | 1750606 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g153800 | GPI8,PIGK1,PIGK | (1 of 1) K05290 - phosphatidylinositol glycan, class K (PIGK); GPI transamidase subunit K | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCATCGGCGACAGACATTTCTCCAATTTG |
| Internal bar code: | TCTTAGAAAGGCGACGTTGACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 497 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 89 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAAGGAGGCTGCTAACACG |
| Suggested primer 2: | ACCCAGTCTTGGCCTTACCT |