Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.237057 |
Chromosome: | chromosome_3 |
Location: | 4755699 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre03.g178100 | Lipoxygenase | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | AAGCGCCAGGTGGCCTCCCCATCCCAAGCC |
Internal bar code: | ACATCACTACCGTCGGGGACAC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 193 |
LEAP-Seq percent confirming: | 83.5821 |
LEAP-Seq n confirming: | 56 |
LEAP-Seq n nonconfirming: | 11 |
LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GGCCCACGAACTACAAGGTA |
Suggested primer 2: | GCGATTACACACTCCCACCT |