| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.237066 |
| Chromosome: | chromosome 6 |
| Location: | 3810621 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278166 | PRL4 | Predicted extracellular protein; (1 of 1) PTHR10334:SF165 - PATHOGENESIS-RELATED PROTEIN-RELATED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTTTTGAGTGTTGCGAGTTGGTTATTGTC |
| Internal bar code: | GGTCGACCCCCCAAGGGTGGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 622 |
| LEAP-Seq percent confirming: | 97.8767 |
| LEAP-Seq n confirming: | 1890 |
| LEAP-Seq n nonconfirming: | 41 |
| LEAP-Seq n unique pos: | 54 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCTGCGGCTATAAGCTATG |
| Suggested primer 2: | TGAGAGCATCCGTCTGTGTC |