| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.237080 |
| Chromosome: | chromosome 16 |
| Location: | 3829063 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g689800 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACGCGCGGAATCATGGGGGGTCTTTCGGC |
| Internal bar code: | TAACCTCTCTATTAGAGTAGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 798 |
| LEAP-Seq percent confirming: | 98.8316 |
| LEAP-Seq n confirming: | 9643 |
| LEAP-Seq n nonconfirming: | 114 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTATTTCACTAGCGAGCGGC |
| Suggested primer 2: | CCTGTGCTGTGTGAAGTCGT |