| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.237082 |
| Chromosome: | chromosome 12 |
| Location: | 4813904 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g524500 | RMT2 | Putative protein N-methyltransferase; (1 of 2) PTHR13271//PTHR13271:SF30 - UNCHARACTERIZED PUTATIVE METHYLTRANSFERASE // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTGTGACTGTGGCTGTGTGTGTTGTGTGT |
| Internal bar code: | TGAGGAGCCGGTCCTCGGGTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 514 |
| LEAP-Seq percent confirming: | 33.119 |
| LEAP-Seq n confirming: | 721 |
| LEAP-Seq n nonconfirming: | 1456 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTACTCCCATTGCCAAACG |
| Suggested primer 2: | CTGTTGTTGTCCTGTGTGGG |