| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.237082 |
| Chromosome: | chromosome 12 |
| Location: | 7757388 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g553800 | (1 of 1) IPR002110//IPR020683//IPR029071 - Ankyrin repeat // Ankyrin repeat-containing domain // Ubiquitin-related domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGCCCCAGGCTACGGTCCACATTGCGCT |
| Internal bar code: | ATCACGCTACACCCCGGTTGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 619 |
| LEAP-Seq percent confirming: | 97.9272 |
| LEAP-Seq n confirming: | 16441 |
| LEAP-Seq n nonconfirming: | 348 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGTACGGCGACTTCTACGC |
| Suggested primer 2: | CAACGACTGGTACGGCAAC |