Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.237094 |
Chromosome: | chromosome_3 |
Location: | 2910089 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre03.g162900 | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TGTTTCTGCCGTGGATTGGAGGCATTGTCG |
Internal bar code: | TTATTATGGTAGCATCTGCAAG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1132 |
LEAP-Seq percent confirming: | 99.286 |
LEAP-Seq n confirming: | 8900 |
LEAP-Seq n nonconfirming: | 64 |
LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GACTCGTGTACCCTGGGTGT |
Suggested primer 2: | CGCGTCAGACTCGATTAACA |