| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.237144 |
| Chromosome: | chromosome 3 |
| Location: | 4864598 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g179300 | SRH6 | (1 of 1) K11367 - chromodomain-helicase-DNA-binding protein 1 [EC:3.6.4.12] (CHD1); SNF2-related DNA/RNA helicase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATTCTCAGAAACCAGCAGATGGCAAACAA |
| Internal bar code: | ACTGGGTTCCTCGGCAGTACCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 666 |
| LEAP-Seq percent confirming: | 96.7572 |
| LEAP-Seq n confirming: | 53319 |
| LEAP-Seq n nonconfirming: | 1787 |
| LEAP-Seq n unique pos: | 263 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCTGACACATGACAGGGTA |
| Suggested primer 2: | TGTGTGAGGAGCACAAGGAG |