| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.237172 |
| Chromosome: | chromosome 12 |
| Location: | 1053881 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g491000 | (1 of 14) PF04577 - Protein of unknown function (DUF563) (DUF563) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTTGAGCCCGATGGGCCGCCAGTGCCGGA |
| Internal bar code: | GTCAAGGGGCCAGCAAATACAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 608 |
| LEAP-Seq percent confirming: | 98.3952 |
| LEAP-Seq n confirming: | 3311 |
| LEAP-Seq n nonconfirming: | 54 |
| LEAP-Seq n unique pos: | 45 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGACTCCAGAGCTGTTTTGC |
| Suggested primer 2: | TGATGGTAACCCGTCCGTAT |