| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.237190 |
| Chromosome: | chromosome 4 |
| Location: | 2689658 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g223250 | LCIE,LCIE1 | Low-CO2 inducible protein E | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGGCTAAGCTGGGGCTTTGGCTGGGGCT |
| Internal bar code: | ACGTCATATATATTAGCAGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 161 |
| LEAP-Seq percent confirming: | 99.3631 |
| LEAP-Seq n confirming: | 1092 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGAGGATGGAGTACTCGGG |
| Suggested primer 2: | ACAACAACTTTTCCAACCGC |