| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.237200 |
| Chromosome: | chromosome 10 |
| Location: | 1949591 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g432250 | (1 of 52) 2.7.11.22 - Cyclin-dependent kinase / Cdk-activating protein kinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACAGACCAGTGCAGTCTGCGCACACACAGT |
| Internal bar code: | GTTTGCGGGAGTGGCGATGAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 481 |
| LEAP-Seq percent confirming: | 99.3252 |
| LEAP-Seq n confirming: | 2355 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCAACCATGTGTTTGACCA |
| Suggested primer 2: | CAGGTTCTTTTTGCACAGCA |