| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.237205 |
| Chromosome: | chromosome 3 |
| Location: | 5258974 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g183200 | CPC1 | Central pair microtubule complex 1; (1 of 1) PTHR14919:SF0 - SPERM FLAGELLAR PROTEIN 2 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCGCTCCAACATGCGCTTCACGAACCGCT |
| Internal bar code: | ACGACGCGACGGACGTCTGACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 127 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 62 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGTACGGGCTCAGAGGAAG |
| Suggested primer 2: | TTAATGCAAGCATCCTGCTG |