| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.237215 |
| Chromosome: | chromosome 16 |
| Location: | 6053126 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g676250 | MFT28 | Major facilitator superfamily transporter; (1 of 2) PTHR23121:SF9 - SODIUM-DEPENDENT GLUCOSE TRANSPORTER 1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGAGTACATCAACGAAATGTCACCCACAA |
| Internal bar code: | CGTTGCAAGCACTATCTTCTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1101 |
| LEAP-Seq percent confirming: | 99.2995 |
| LEAP-Seq n confirming: | 11908 |
| LEAP-Seq n nonconfirming: | 84 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGACCTACGCGCTAACCAG |
| Suggested primer 2: | CCAAGCTTCTTAAACTGCCG |