Insertion junction: LMJ.RY0402.237215_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.237215
Chromosome:chromosome 16
Location:6053127
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre16.g676250 MFT28 Major facilitator superfamily transporter; (1 of 2) PTHR23121:SF9 - SODIUM-DEPENDENT GLUCOSE TRANSPORTER 1 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTTCCGCCTACAGTTCGCACCCCCTGGCC
Internal bar code:CGAGTCGTCAGTGACCCTGCAG

Confirmation - LEAP-Seq

LEAP-Seq distance:872
LEAP-Seq percent confirming:99.411
LEAP-Seq n confirming:8270
LEAP-Seq n nonconfirming:49
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:CCAAGCTTCTTAAACTGCCG
Suggested primer 2:GAGACCTACGCGCTAACCAG