| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.237240 |
| Chromosome: | chromosome 4 |
| Location: | 2959675 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g224826 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGGAGGTGCGTGTGTGGGTGGGTGTGGG |
| Internal bar code: | ACGTCGAAATGGATGACATACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 693 |
| LEAP-Seq percent confirming: | 97.2762 |
| LEAP-Seq n confirming: | 7714 |
| LEAP-Seq n nonconfirming: | 216 |
| LEAP-Seq n unique pos: | 46 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTTAGCCCTCCCACGTTTT |
| Suggested primer 2: | AACATGCGTATGCGGTATCA |