| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.237246 |
| Chromosome: | chromosome 9 |
| Location: | 1287014 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g399150 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTTGACTGCATAGCAGCAGCTCGCTATG |
| Internal bar code: | TTTCTGGTTACTCTACTTCTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1139 |
| LEAP-Seq percent confirming: | 83.5955 |
| LEAP-Seq n confirming: | 116049 |
| LEAP-Seq n nonconfirming: | 22773 |
| LEAP-Seq n unique pos: | 124 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGTACGATGGTGTGTAACG |
| Suggested primer 2: | TGGTTGAGGGGAACTGAAAC |