| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.237267 |
| Chromosome: | chromosome 16 |
| Location: | 1362704 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g651900 | CRI1,OPR129,OPR126,CRTISO | OctotricoPeptide Repeat protein 129 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGCCGCCGCCACGTCCGCGACCGAACTGT |
| Internal bar code: | GACAGCAGACTTCGTTAGATCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 862 |
| LEAP-Seq percent confirming: | 98.4252 |
| LEAP-Seq n confirming: | 1125 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCGAGATGTGTGTGTGTG |
| Suggested primer 2: | GCCCCATCATTATAGAGCGA |