Insertion junction: LMJ.RY0402.237267_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.237267
Chromosome:chromosome 16
Location:1362711
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre16.g651900 CRI1,OPR129,OPR126,CRTISO OctotricoPeptide Repeat protein 129 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTCGTGGCTGACAGCCGAGGCGCTGAGGC
Internal bar code:CCTCCCCCGAATCGAAGGTGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:423
LEAP-Seq percent confirming:92.6829
LEAP-Seq n confirming:38
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:CTGCGAGATGTGTGTGTGTG
Suggested primer 2:GCCCCATCATTATAGAGCGA