| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.237268 |
| Chromosome: | chromosome 6 |
| Location: | 1652218 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g261000 | PSBR1,PSBR | (1 of 1) K03541 - photosystem II 10kDa protein (psbR); Photosystem II 10 kDa polypeptide | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTGGGCAGTAGCCCCCGTCAGTGCCTAGT |
| Internal bar code: | GTTACTAGCGCCTGGCCGCAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1052 |
| LEAP-Seq percent confirming: | 99.5885 |
| LEAP-Seq n confirming: | 10165 |
| LEAP-Seq n nonconfirming: | 42 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATGTTAAGGAGTCAGCGCA |
| Suggested primer 2: | TAGCTCTCCGCTCTTTCTGC |