| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.237271 |
| Chromosome: | chromosome 7 |
| Location: | 824013 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g318350 | CGL64 | (1 of 4) PF12576 - Protein of unknown function (DUF3754) (DUF3754); Conserved in the Green Lineage | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGAAGGAACAGTGGGCTGCGCCCACGCCA |
| Internal bar code: | CAATTCGGCGTCATTTTATAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1082 |
| LEAP-Seq percent confirming: | 98.9953 |
| LEAP-Seq n confirming: | 7587 |
| LEAP-Seq n nonconfirming: | 77 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATGGTTGGTGGTAGTGCTG |
| Suggested primer 2: | GGCCCAATTGAACTCTTGAA |