Insertion junction: LMJ.RY0402.237272_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.237272
Chromosome:chromosome 11
Location:3261627
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre11.g479850 CYN59 Cyclophilin 59; (1 of 1) K12735 - peptidyl-prolyl cis-trans isomerase-like 4 [EC:5.2.1.8] (PPIL4) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCACCACACACACCACCACGGTGCACCGTG
Internal bar code:TTTCCTCGCTTTATGCTAGGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:471
LEAP-Seq percent confirming:99.4009
LEAP-Seq n confirming:2157
LEAP-Seq n nonconfirming:13
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:GACCTGCTGCTGATGGATG
Suggested primer 2:TCGGTCATGAACAACCAAAA