Insertion junction: LMJ.RY0402.237272_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.237272
Chromosome:chromosome 11
Location:3261630
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre11.g479850 CYN59 Cyclophilin 59; (1 of 1) K12735 - peptidyl-prolyl cis-trans isomerase-like 4 [EC:5.2.1.8] (PPIL4) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTGTTGCCCGTGCGGCCGAACGGACTTGC
Internal bar code:GCACTGTGGGTGACACTCCTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:635
LEAP-Seq percent confirming:98.852
LEAP-Seq n confirming:775
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:GACCTGCTGCTGATGGATG
Suggested primer 2:TCGGTCATGAACAACCAAAA