| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.237279 |
| Chromosome: | chromosome 8 |
| Location: | 3215369 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g373500 | (1 of 1) K10308 - F-box protein 31 (FBXO31) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCGCGCAGGTGGGTGTGCGACTAGGCGGC |
| Internal bar code: | TCTTAATATTTCGTGCGTCACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 789 |
| LEAP-Seq percent confirming: | 99.8679 |
| LEAP-Seq n confirming: | 756 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAGGCAGTGGTAGAAGCAG |
| Suggested primer 2: | CTTTCGCTTTTGCATCCTTC |