| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.237293 |
| Chromosome: | chromosome 1 |
| Location: | 4208680 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g027950 | IFT74,CMG1,IFT71,IFT72 | (1 of 1) PTHR31432:SF0 - INTRAFLAGELLAR TRANSPORT PROTEIN 74 HOMOLOG; Intraflagellar transport protein 74/72 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATGGGAGTAGGCTAGGCCTGTGTGAGGGC |
| Internal bar code: | GGGCAATGTCGAGGCGCCACTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 584 |
| LEAP-Seq percent confirming: | 98.6626 |
| LEAP-Seq n confirming: | 2582 |
| LEAP-Seq n nonconfirming: | 35 |
| LEAP-Seq n unique pos: | 50 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACATGTTGGTTGTGCAGGAA |
| Suggested primer 2: | CGATTTCGTCTTGCAACTCA |