| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.237297 |
| Chromosome: | chromosome_3 |
| Location: | 3578947 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre03.g168200 | FAP69 | Flagellar Associated Protein | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGGAAGCTCTGCAGGCTGTTTTTGAGCATG |
| Internal bar code: | TGTTACTCCTTAGGCGTTGCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 527 |
| LEAP-Seq percent confirming: | 99.4852 |
| LEAP-Seq n confirming: | 53913 |
| LEAP-Seq n nonconfirming: | 279 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGAAGTACGCCAAGTTCCGA |
| Suggested primer 2: | TGCACATGACTAGCAGGAGG |