| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.237301 |
| Chromosome: | chromosome 1 |
| Location: | 4726110 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g032550 | CGL127 | (1 of 1) K11267 - sister chromatid cohesion protein PDS5 (PDS5); Conserved in the Green Lineage | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAAACGCCTCGGCCTGAACTGCAATGTCTT |
| Internal bar code: | ACGGGGGGCTTACCCTAACAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1083 |
| LEAP-Seq percent confirming: | 99.3167 |
| LEAP-Seq n confirming: | 13227 |
| LEAP-Seq n nonconfirming: | 91 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCGACTCCTCTATCATGCC |
| Suggested primer 2: | TTCACGCAGCTGACCTTATG |