| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.237304 |
| Chromosome: | chromosome 12 |
| Location: | 1494389 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g487400 | (1 of 2) PF03556//PF14555 - Cullin binding (Cullin_binding) // UBA-like domain (UBA_4) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCACTTGCAGCCGCCTCGTTCTTAGCCCC |
| Internal bar code: | TTGATAATTAAAGAGAGTTCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 754 |
| LEAP-Seq percent confirming: | 99.9074 |
| LEAP-Seq n confirming: | 3238 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCGTACTGCATGAGGGAAC |
| Suggested primer 2: | AAGTACTGTGGGACGTTGGC |