Insertion junction: LMJ.RY0402.237312_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.237312
Chromosome:chromosome 6
Location:7368674
Confidence (%):58
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGGTGGCCACTCTGACGGGCTTCGAGTAAC
Internal bar code:TGGCTTTTTCCTCGCAGTGGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:982
LEAP-Seq percent confirming:99.6339
LEAP-Seq n confirming:2449
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:CCGTGTTGTTTGGATGTCAG
Suggested primer 2:TAAGTACCCGGCAAAACCTG