| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.237316 |
| Chromosome: | chromosome 13 |
| Location: | 317365 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g564200 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCAAGTTGACACTCTTTGCTAGTTGTACT |
| Internal bar code: | ATGTGGTCCCGAACACCAGCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 611 |
| LEAP-Seq percent confirming: | 99.0917 |
| LEAP-Seq n confirming: | 2182 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 39 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGTTTGCTTTGATGCCTGA |
| Suggested primer 2: | GAAGGCACTTCCAATGTGGT |