| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.237325 |
| Chromosome: | chromosome 12 |
| Location: | 5089044 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g526700 | RBL9 | Rhomboid-like protease; (1 of 11) 3.4.21.105 - Rhomboid protease | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCGGATGAACCCCTATCCATGCCGTATGT |
| Internal bar code: | TGGTTTGCCCGTGCGTGTCCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 156 |
| LEAP-Seq percent confirming: | 99.2018 |
| LEAP-Seq n confirming: | 870 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGGCTGTGTGCTGTTTTGT |
| Suggested primer 2: | TGTTTGTGTGTGGGTGTGTG |