| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.237326 |
| Chromosome: | chromosome 12 |
| Location: | 7731530 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g553900 | CNG2 | (1 of 1) K04905 - potassium voltage-gated channel Eag-related subfamily H member 2 (KCNH2); Cyclic-nucleotide gated potassium channel | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTAAGCCTAGGCACCAACGGTCTCTCTCTC |
| Internal bar code: | GCAGGTTATCCCAGTGGGGCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 583 |
| LEAP-Seq percent confirming: | 98.4031 |
| LEAP-Seq n confirming: | 8504 |
| LEAP-Seq n nonconfirming: | 138 |
| LEAP-Seq n unique pos: | 97 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAAGATCTTCCGCATTGTT |
| Suggested primer 2: | GCGCTTCTTTAACAGTTGCC |