Insertion junction: LMJ.RY0402.237330_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.237330
Chromosome:chromosome 3
Location:5251710
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g183150 (1 of 1) 3.4.24.21 - Astacin / Crayfish small-molecule proteinase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGTTTAGAATGTGTCCCGGGCATGTGGCAC
Internal bar code:CCACTAGGTGGATACTGCTAAA

Confirmation - LEAP-Seq

LEAP-Seq distance:0
LEAP-Seq percent confirming:0.00578704
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:17279
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CCCTGTCCTAAACTCCCACA
Suggested primer 2:GGGTGACGAGTTTTCGTTGT